Cocaine‐ and amphetamine‐regulated transcript peptide (CART) in the telencephalon of the catfish, Clarias gariepinus: Distribution and response to fasting, 2‐deoxy‐D‐glucose, glucose, insulin, and leptin treatments
Identifieur interne : 000349 ( Main/Exploration ); précédent : 000348; suivant : 000350Cocaine‐ and amphetamine‐regulated transcript peptide (CART) in the telencephalon of the catfish, Clarias gariepinus: Distribution and response to fasting, 2‐deoxy‐D‐glucose, glucose, insulin, and leptin treatments
Auteurs : Nishikant Subhedar [Inde] ; Vikas G. Barsagade [Inde] ; Praful S. Singru [Inde] ; Lars Thim [Danemark] ; Jes Thorn Clausen [Danemark]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2011-05-01.
English descriptors
- KwdEn :
Abstract
The cocaine‐ and amphetamine‐regulated transcript peptide (CART)‐containing system in the forebrain of Clarias gariepinus was studied with immunocytochemistry. While the immunoreactivity was prominently seen in the neurons of the entopeduncular nucleus (EN) located in the ventral telencephalon, CART‐immunoreactive fibers were widely distributed in the dorsal and ventral telencephalon. In view of the established role of CART in energy metabolism, we investigated the response of the CART immunoreactive system to positive and negative nutritional conditions. Neurons of the EN and fibers in the different areas of the telencephalon showed significant reduction in CART immunoreactivity following 48 hours food deprivation, or 2 hours following intracranial administration of 2‐deoxy‐D‐glucose (2DG, 100 ng/g body weight, a metabolic antagonist of glucose). However, intracranial injection of glucose (100 ng/g body weight) resulted in a distinct increase in CART immunoreactivity in these components. In mammals, insulin and leptin have been recognized as adiposity agents that convey peripheral energy status‐related information to brain. Intracranial administration of insulin (3 mU/fish) and leptin (10 ng/g body weight) significantly increased CART immunoreactivity in the EN neurons and in the fiber network within 2 hours. Superfusion of the EN‐containing tissue fragments in the medium enriched in glucose, insulin, or leptin evoked a significant increase in CART immunoreactivity in the EN neurons, but 2DG reduced the immunoreactivity. We suggest that CART‐containing neurons of the EN, and fibers in the telencephalon, may process the energy status‐related information and contribute to satiety. J. Comp. Neurol. 519:1281–1300, 2011. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/cne.22569
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000489
- to stream Istex, to step Curation: 000489
- to stream Istex, to step Checkpoint: 000118
- to stream Main, to step Merge: 000352
- to stream Main, to step Curation: 000349
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cocaine‐ and amphetamine‐regulated transcript peptide (CART) in the telencephalon of the catfish, Clarias gariepinus: Distribution and response to fasting, 2‐deoxy‐D‐glucose, glucose, insulin, and leptin treatments</title><author><name sortKey="Subhedar, Nishikant" sort="Subhedar, Nishikant" uniqKey="Subhedar N" first="Nishikant" last="Subhedar">Nishikant Subhedar</name></author><author><name sortKey="Barsagade, Vikas G" sort="Barsagade, Vikas G" uniqKey="Barsagade V" first="Vikas G." last="Barsagade">Vikas G. Barsagade</name></author><author><name sortKey="Singru, Praful S" sort="Singru, Praful S" uniqKey="Singru P" first="Praful S." last="Singru">Praful S. Singru</name></author><author><name sortKey="Thim, Lars" sort="Thim, Lars" uniqKey="Thim L" first="Lars" last="Thim">Lars Thim</name></author><author><name sortKey="Clausen, Jes Thorn" sort="Clausen, Jes Thorn" uniqKey="Clausen J" first="Jes Thorn" last="Clausen">Jes Thorn Clausen</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:DCA66A14500569A46052131E11B950B6C545AAFB</idno><date when="2011" year="2011">2011</date><idno type="doi">10.1002/cne.22569</idno><idno type="url">https://api.istex.fr/document/DCA66A14500569A46052131E11B950B6C545AAFB/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000489</idno><idno type="wicri:Area/Istex/Curation">000489</idno><idno type="wicri:Area/Istex/Checkpoint">000118</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000118</idno><idno type="wicri:doubleKey">0021-9967:2011:Subhedar N:cocaine:and:amphetamine</idno><idno type="wicri:Area/Main/Merge">000352</idno><idno type="wicri:Area/Main/Curation">000349</idno><idno type="wicri:Area/Main/Exploration">000349</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Cocaine‐ and amphetamine‐regulated transcript peptide (CART) in the telencephalon of the catfish, Clarias gariepinus: Distribution and response to fasting, 2‐deoxy‐D‐glucose, glucose, insulin, and leptin treatments</title><author><name sortKey="Subhedar, Nishikant" sort="Subhedar, Nishikant" uniqKey="Subhedar N" first="Nishikant" last="Subhedar">Nishikant Subhedar</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Indian Institute of Science Education and Research, Sutarwadi, Pashan, Pune 411 021</wicri:regionArea><wicri:noRegion>Pune 411 021</wicri:noRegion></affiliation></author><author><name sortKey="Barsagade, Vikas G" sort="Barsagade, Vikas G" uniqKey="Barsagade V" first="Vikas G." last="Barsagade">Vikas G. Barsagade</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Pharmaceutical Sciences, R.T.M. Nagpur University Campus, Nagpur‐440 033</wicri:regionArea><wicri:noRegion>Nagpur‐440 033</wicri:noRegion></affiliation></author><author><name sortKey="Singru, Praful S" sort="Singru, Praful S" uniqKey="Singru P" first="Praful S." last="Singru">Praful S. Singru</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>School of Biological Sciences, National Institute of Science Education and Research (NISER), Institute of Physics Campus, Sachivalaya Marg, PO Sainik School, Bhubaneswar 751 005</wicri:regionArea><wicri:noRegion>Bhubaneswar 751 005</wicri:noRegion></affiliation></author><author><name sortKey="Thim, Lars" sort="Thim, Lars" uniqKey="Thim L" first="Lars" last="Thim">Lars Thim</name><affiliation wicri:level="1"><country xml:lang="fr">Danemark</country><wicri:regionArea>Department of Protein Chemistry, Novo Nordisk A/S, DK‐2880 Bagsvaerd</wicri:regionArea><wicri:noRegion>DK‐2880 Bagsvaerd</wicri:noRegion></affiliation></author><author><name sortKey="Clausen, Jes Thorn" sort="Clausen, Jes Thorn" uniqKey="Clausen J" first="Jes Thorn" last="Clausen">Jes Thorn Clausen</name><affiliation wicri:level="1"><country xml:lang="fr">Danemark</country><wicri:regionArea>Department of Assay and Cell Technology, Novo Nordisk A/S, DK‐2880 Bagsvaerd</wicri:regionArea><wicri:noRegion>DK‐2880 Bagsvaerd</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2011-05-01">2011-05-01</date><biblScope unit="volume">519</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1281">1281</biblScope><biblScope unit="page" to="1300">1300</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">DCA66A14500569A46052131E11B950B6C545AAFB</idno><idno type="DOI">10.1002/cne.22569</idno><idno type="ArticleID">CNE22569</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>entopeduncular nucleus</term><term>fish brain</term><term>insulin</term><term>leptin</term><term>telencephalon</term><term>teleost</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="fr">The cocaine‐ and amphetamine‐regulated transcript peptide (CART)‐containing system in the forebrain of Clarias gariepinus was studied with immunocytochemistry. While the immunoreactivity was prominently seen in the neurons of the entopeduncular nucleus (EN) located in the ventral telencephalon, CART‐immunoreactive fibers were widely distributed in the dorsal and ventral telencephalon. In view of the established role of CART in energy metabolism, we investigated the response of the CART immunoreactive system to positive and negative nutritional conditions. Neurons of the EN and fibers in the different areas of the telencephalon showed significant reduction in CART immunoreactivity following 48 hours food deprivation, or 2 hours following intracranial administration of 2‐deoxy‐D‐glucose (2DG, 100 ng/g body weight, a metabolic antagonist of glucose). However, intracranial injection of glucose (100 ng/g body weight) resulted in a distinct increase in CART immunoreactivity in these components. In mammals, insulin and leptin have been recognized as adiposity agents that convey peripheral energy status‐related information to brain. Intracranial administration of insulin (3 mU/fish) and leptin (10 ng/g body weight) significantly increased CART immunoreactivity in the EN neurons and in the fiber network within 2 hours. Superfusion of the EN‐containing tissue fragments in the medium enriched in glucose, insulin, or leptin evoked a significant increase in CART immunoreactivity in the EN neurons, but 2DG reduced the immunoreactivity. We suggest that CART‐containing neurons of the EN, and fibers in the telencephalon, may process the energy status‐related information and contribute to satiety. J. Comp. Neurol. 519:1281–1300, 2011. © 2010 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Danemark</li><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Subhedar, Nishikant" sort="Subhedar, Nishikant" uniqKey="Subhedar N" first="Nishikant" last="Subhedar">Nishikant Subhedar</name></noRegion><name sortKey="Barsagade, Vikas G" sort="Barsagade, Vikas G" uniqKey="Barsagade V" first="Vikas G." last="Barsagade">Vikas G. Barsagade</name><name sortKey="Singru, Praful S" sort="Singru, Praful S" uniqKey="Singru P" first="Praful S." last="Singru">Praful S. Singru</name></country><country name="Danemark"><noRegion><name sortKey="Thim, Lars" sort="Thim, Lars" uniqKey="Thim L" first="Lars" last="Thim">Lars Thim</name></noRegion><name sortKey="Clausen, Jes Thorn" sort="Clausen, Jes Thorn" uniqKey="Clausen J" first="Jes Thorn" last="Clausen">Jes Thorn Clausen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Eau/explor/LotaV3/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000349 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000349 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Eau
|area= LotaV3
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:DCA66A14500569A46052131E11B950B6C545AAFB
|texte= Cocaine‐ and amphetamine‐regulated transcript peptide (CART) in the telencephalon of the catfish, Clarias gariepinus: Distribution and response to fasting, 2‐deoxy‐D‐glucose, glucose, insulin, and leptin treatments
}}
| This area was generated with Dilib version V0.6.39. |  |